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gpsB [2018-09-07 13:27:45]
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gpsB [2018-09-07 13:27:45]

required for removal of PBP1 from the cell pole after completion of cell pole maturation
locus
BSU22180
pI
5.63
mw
11.45 kDa
protein length
98 aa Sequence Blast
gene length
294 bp Sequence Blast
function
cell division, cell elongation
product
cell division protein, member of the divisome
essential
no
synonyms
ypsB
Outlinks
KEGGBsubCycSubtiListExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

    • Gene

    Coordinates
    2,331,779 → 2,332,075

    Phenotypes of a mutant

  • synthetic lethal phenotype with a ''[gene|672EA84D7725BE21F649DF30A11EB4E0EDFC3925|ftsA]'' mutation [Pubmed|18776011]; synthetic sick phenotype when combined with an ''[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' mutation [Pubmed|18363795], although this seems controversial [Pubmed|18776011]
  • reduced growth at high salt [Pubmed|25845974]

    • The protein

    Catalyzed reaction/ biological activity

  • activates [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC] [Pubmed|25845974]

    • Paralogous protein(s)

  • [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|DivIVA]

    • [SW|Domains]

  • contains a [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|DivIVA]-like N-terminal domain

    • Modification

  • phosphorylation on Thr-75 by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC] [Pubmed|25845974,17218307]

    • Structure

  • [PDB|4UG3] (N-terminal domain, aa 1-68) [Pubmed|26575090]
  • [PDB|5AN5] (C-terminal domain, aa 76-98) [Pubmed|26575090]
  • GpsB is hexameric (trimer of dimers) [Pubmed|26575090]

    • [SW|Localization]

  • dynamic, shuttles between lateral membrane and the divison septum [Pubmed|18363795], localizes to the divisome after Z-ring formation [Pubmed|18776011]

    • Expression and Regulation

    Operons

    genes
    [gene|C22F7704DC9D36D96FE089ADCEA546D7A3FB3739|gpsB]-[gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]-[gene|0DF27BEAF763545258CE7DD56F65CD5BF74DD887|ypsC]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A472 (gpsB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/472 NBRP B. subtilis, Japan]
  • 1A934 ( ''gpsB''::''kan''), [Pubmed|18776011], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A934&Search=1A934 BGSC]
  • 1A970 ( ''gpsB''::''kan''), [Pubmed|18776011], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A970&Search=1A970 BGSC]
  • BKE22180 (Δ[gene|C22F7704DC9D36D96FE089ADCEA546D7A3FB3739|gpsB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTCACCTCGTATC, downstream forward: _UP4_TGATCACGCTTGAAAAAAAT
  • BKK22180 (Δ[gene|C22F7704DC9D36D96FE089ADCEA546D7A3FB3739|gpsB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTCACCTCGTATC, downstream forward: _UP4_TGATCACGCTTGAAAAAAAT

    • GFP fusion

  • [Pubmed|18363795,18776011]

    • two-hybrid system

  • [Pubmed|18363795]

    • Antibody

  • raised against L. monocytogenes GpsB [Pubmed|26575090] available in [SW|Sven Halbedel] 's lab

    • References

  • 18776011,25845974,19429628,17218307,18363795,26575090,27257764